Studies on Polymyxin: The Production of Fermentation Liquor

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Abstract
The production of polymyxin fermentation liquor on a large laboratory scale is described and the details of an assembly suitable for this purpose given. The medium consists of 2% (NH4)2SO4, 1% glucose, 0.5% yeast extract, 0.2% KH2PO4, 0.05% MgSO4 7H2O, 0.005% NaCl, and 0.001% FeSO4.-7H2O, pH 7. Incubation is at 25 C for 3 days. Shallow layer production (e.g., 100 ml. per 500-ml. Erlenmeyer flask) gives satisfactory yields. Deep.fermentation requires moderate aeration. 15 liters of medium in a 22-liter round-bottom flask was aerated at the rate of 64 1. per hr. Foaming was controlled by the addition of 15 ml. of a soln. of 1% octa-decanol in mineral oil at the start of the fermentation. The average potency obtained was about 200 U./ml.
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