Recovery of Chlamydia trachomatis from Patients of a Southeastern Venereal Disease Clinic

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Abstract
The authors determined the incidences of Chlamydia trachomatis colonization obtained by use of two methods of isolation in 169 specimens from individuals attending a venereal disease clinic. Specimens were collected in phosphate sucrose buffer and planted simultaneously onto McCoy cells previously treated with 5-iodo-2-deoxyuridine (IUDR) and with cycloheximide. As controls, specimens were obtained from 76 hospital employees or medical students without clinical signs of infection and cultured in a similar manner. Qualitatively, recoveries of Chlamydia trachomatis inclusions from the differently treated cells were the same, but the number of inclusions per ml was greater for the cycloheximide-treated cells. Recoveries of Chlamydia trachomatis from the venereal disease clinic population were 33% for those patients who also had simultaneous cultures positive for Neisseria gonorrhoeae and 42% for those with nongonococcal urethritis. The recovery of Chlamydia trachomatis from the control group was 5%.

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