Abbau von Steroiden bei der Inkubation mit β-Glucuronidase and während der Lagerung von Urinen

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Abstract
Androsterone, etiocholanolone, pregnanetriol, dehydroepiandrosterone, pregnanediol, tetrahydrocortisol, 5-pregnenolone and 11-.beta.-OH-androsterone were incubated with .beta.-glucuronidase preparations (Helix pomatia, bovine liver and Escherichia coli) for 96 h at 37.degree. C. After extraction and silylation they were gas-chromatographed. The 1st 3 steroids were left practically intact. The least decomposition of the last 5 steroids occurred with the liver enzyme. Testosterone and 11-ketoandrosterone without the enzymes showed 74 and 35% recoveries. Cortisol and tetrahydrocortisol, incubated with the 1st 2 enzymes for 18 h at 37.degree. C and 48.degree. C, showed nearly 100% recoveries. The recoveries of 17-hydroxycorticosteroids in (human) urines (pH 7.8-8.8), stored for 7 days, was 80% at 20.degree.-25.degree. C and 55% at 25.degree.-30.degree. C. The same samples, brought to pH 1.8-2.8 with NaHSO4 before the storage, showed 100% recovery.

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