A Simplified Acid Hematein Test for Phospholipids

Publisher Website
Google Scholar
Abstract
The acid hematein test of Baker was simplified by the following method: Tissues were fixed in either formal-calcium or formal-calcium-cadmium, frozen-sectioned in a cryostat, cbromatized with potassium dichromate, washed in water, stained in acid hematein solution, washed in water again, differentiated in borax-ferricyanide, dehydrated in alcohols, cleared in xylene, and finally mounted in balsam. The process requires only 2 days instead of the 6 days necessary for the original test of Baker. The technique stains phospholipid in mitochondria and in other cellular elements, and is recommended especially for mitochondria

This publication has 8 references indexed in Scilit: