Identification of cholesterol‐bound aldehydes in copper‐oxidized low density lipoprotein

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Abstract
Lipid-soluble cholesteryl ester core aldehydes (aldehydes still bound to the cholesterol ring) were identified among the products of copper-catalyzed peroxidation of human low density lipoprotein (LDL). The LDL was exposed to oxygenated buffer and 5 μM CuSO4 for 24 h. The core aldehydes were isolated as the dinitrophenylhydrazones, and were identified by reverse-phase HPLC with mass spectrometry. The major components were the C4–C10 oxoalkanoyl esters of cholesterol and 7-ketecholesterol, and accounted for 1–2% of the cholesteryl linoleate and arachidonate consumed.