The exocyst complex binds the small GTPase RalA to mediate filopodia formation
- 17 December 2001
- journal article
- research article
- Published by Springer Nature in Nature Cell Biology
- Vol. 4 (1) , 73-78
- https://doi.org/10.1038/ncb720
Abstract
The Ras-related small GTPase RalA is involved in controlling actin cytoskeletal remodelling and vesicle transport in mammalian cells1,2. We identified the mammalian homologue of Sec5, a subunit of the exocyst complex determining yeast cell polarity, as a specific binding partner for GTP-ligated RalA. Inhibition of RalA binding to Sec5 prevents filopod production by tumor necrosis factor-α (TNF-α) and interleukin-1 (IL-1) and by activated forms of RalA and Cdc42, signalling intermediates downstream of these inflammatory cytokines. We propose that the RalA–exocyst complex interaction integrates the secretory and cytoskeletal pathways.Keywords
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