Separation of collagen cyanogen bromide-derived peptides by reversed-phase high-performance liquid chromatography
- 1 October 1980
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 191 (1) , 253-256
- https://doi.org/10.1042/bj1910253
Abstract
A new technique for separation of the CNBr cleavage products of collagen is described. It involves the use of a 30 nm pore reversed-phase high-performance liquid chromatography column eluted with a linear gradient of acetonitrile/water containing 0.01 M-heptafluorobutyric acid. The separation of type I, type II and type III CNBr peptides is described. Resolution is particularly good for the low-molecular-weight peptides. The method is fast, quantitative and sensitive, and the complete volatility of the eluent facilitates the recovery of the separated peptides.Keywords
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