Total Length Reduction in Preserved Yellow Perch Larvae

Abstract
The high variability in reported lengths of larval yellow perch Perca flavescens at hatching, dietary shift, and morphometric transformation may be partly caused by shrinkage that occurs after preservation. Larval yellow perch were captured, randomly assigned to one of six preservative treatments (100%, 95%, 80%, and 50% ethyl alcohol and 5% and 10% formalin), and measured (before preservation) for total length (TL). Larval yellow perch total lengths were then recorded on days 1, 7, 14, and 21 after storage in each of the six preservatives. Significant reductions in TL (11.5–14.3%) occurred during the first 24 h after fixation and larvae continued to contract at a lesser rate through day 7 in all four ethyl alcohol treatments. Total length reductions of up to 2.5% also occurred during the first 24 h in each formalin concentration. Our findings report the total length reductions of larval yellow perch at a length range used by some biologists when indexing year-class strength and during studies of early life history. The length reductions of larval yellow perch that are associated with storage in different concentrations of preservative appear to be inconsistent with those noted for other species; therefore, our findings support the need to obtain species-specific data for larval shrinkage.
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