Production, purification and molecular weight determination of the haemolysin of Treponema hyodysenteriae
- 1 November 1988
- journal article
- research article
- Published by Microbiology Society in Journal of Medical Microbiology
- Vol. 27 (3) , 215-224
- https://doi.org/10.1099/00222615-27-3-215
Abstract
Summary The production of haemolysin from Treponema hyodysenteriae was increased by an improved culture method and by repeated incubation of spirochaetes suspended in a buffer containing RNA-core. Ion exchange chromatography on DEAE cellulose followed by gel filtration on Sephadex G100 yielded purified haemolysin free from extraneous protein, as judged by silver-stained polyacrylamide gels. The mol. wt of the purified haemolysin, determined by gel filtration was 19 000, a value similar to that of streptolysin S, but much lower than that previously reported.This publication has 11 references indexed in Scilit:
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