Gal3p and Gal1p interact with the transcriptional repressor Gal80p to form a complex of 1:1 stoichiometry
- 1 May 2002
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 363 (3) , 515-20
- https://doi.org/10.1042/0264-6021:3630515
Abstract
The genes encoding the enzymes required for galactose metabolism in Saccharomyces cerevisiae are controlled at the level of transcription by a genetic switch consisting of three proteins: a transcriptional activator, Gal4p; a transcriptional repressor, Gal80p; and a ligand sensor, Gal3p. The switch is turned on in the presence of two small molecule ligands, galactose and ATP. Gal3p shows a high degree of sequence identity with Gal1p, the yeast galactokinase. We have mapped the interaction between Gal80p and Gal3p, which only occurs in the presence of both ligands, using protease protection experiments and have shown that this involves amino acid residue 331 of Gal80p. Gel-filtration experiments indicate that Gal3p, or the galactokinase Gal1p, interact directly with Gal80p to form a complex with 1:1 stoichiometry.Keywords
This publication has 19 references indexed in Scilit:
- Gal80-Gal80 interaction on adjacent Gal4p binding sites is required for complete GAL gene repressionThe EMBO Journal, 2001
- The yeast galactose genetic switch is mediated by the formation of a Gal4p–Gal80p–Gal3p complexThe EMBO Journal, 1998
- Signaling activation and repression of RNA polymerase II transcription in yeastBioEssays, 1997
- Galactose-dependent reversible interaction of Gal3p with Gal80p in the induction pathway of Gal4p-activated genes of Saccharomyces cerevisiaeProceedings of the National Academy of Sciences, 1997
- Activation of Gal4p by Galactose-Dependent Interaction of Galactokinase and Gal80pScience, 1996
- Overproduction and single-step purification of GAL4 fusion proteins from Escherichia coliGene, 1993
- Nucleotide sequence of the yeast regulatory geneGAL80Nucleic Acids Research, 1984
- The organization and transcription of the galactose gene cluster of SaccharomycesJournal of Molecular Biology, 1981
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970