"Fingerprinting" high molecular weight RNA by two-dimensional gel electrophoresis: application to poliovirus RNA
Open Access
- 1 July 1976
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 3 (7) , 1647-1658
- https://doi.org/10.1093/nar/3.7.1647
Abstract
Conditions are described under which complete RNase T1 digests of high molecular weight RNA can be separated into numerous components by two-dimensional gel electrophoresis. Small and large oligonucleotides (n = 1 – 200) can be resolved without losses. The procedure yields fingerprints which are diagnostic for a particular species of RNA and an index of its purity as will be shown for the genomes of poliovirus type 1 and 2.Keywords
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