Nudeotide sequence of the essential region of bacteriophage P4

Open Access
Publisher Website
Google Scholar
Abstract
Nucleotide sequence of one-third of the genome of coliphage P4 has been obtained and mutations vir l. εaml04, c 1405, sid l, and δ35 identified. The ε gene likely encodes a 10 kd protein with εaml04 being located at the beginning of the gene. c I405, a proposed repressor gene mutation, is located in a sequence capable of coding for a 15 kd protein. A new class of P4 mutations, ash , is located in the neighborhood of c I405. Two TATA-like sequences are mapped 5′ to this c I ( ash ) sequence. Vir l is possibly a promoter-up mutation and is located near or within the replication origin, which is about 400 bp long and AT rich. A sid l mutation is amber that shortens the sid protein by 9 amino acids. The δ gene may encode a 17 kd protein and appears to be coupled with the sid gene translationally. In the 5′ side of the sid gene a sequence of CACAAT is the best TATA-like sequence. Sequences of two possible genes that are previously unrecognized and part of the α and psu genes are also identified.