Phytohaemagglutinin‐Induced Leukocyte Migration Inhibition and Phytohaemagglutinin Skin Test for Screening the Cell‐Mediated Immune Response in Children

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Abstract
Phytohemagglutinin-induced leukocyte migration inhibition was studied in 72 children and compared with phytohemagglutinin skin testing. The methods were used for screening the cell-mediated immune response. The in vitro method, which requires only 10-15% of the number of migrating cells as compared with other migration techniques, proved to be more sensitive, but gave some false-positive results when compared with other immunological parameters. Application of the microdroplet leukocyte migration is advantageous when used either simultaneously with the skin test or as a single screening method in prematures, mature newborns and young infants, where application and/or evaluation of the skin tests is difficult. [The method of leukocyte migration inhibition is widely accepted as an in vitro correlate of delayed hypersensitivity].