Growth of Anaerobes in Crystal Violet Bile Media

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Abstract
Peptone-lactose broth containing varying amounts of bile and crystal violet were tested with anaerobes, a number requiring a dye concentration greater than 1: 1000 to inhibit growth. Clostridium welchii, type 11, was most resistant. In bile media without dye, most anaerobes grew well at a bile content of 1-15%. Crystal violet in concentrations over 1:75,000 in lactose broth inhibits most anaerobes except Cl. welchii, type 11. Bile destroys the inhibitive power of crystal violet for most anaerobes.

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