Linking low dispersion liquid chromatography with diode-array detection for the sensitive and selective determination of vitamins A, D and E
- 1 January 1986
- journal article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 111 (6) , 601-604
- https://doi.org/10.1039/an9861100601
Abstract
A low dispersion LC system using narrow-bore column technology was linked to a diode-array detector and applied to the determination of vitamins. The LC system had been evaluated for dispersion effects. The technique was robust and reliable and proved to be extremely sensitive and selective for the determination of vitamins A, D and E. Vitamin E was assayed in various vegetable oils and the percentage distribution examined. The high mass sensitivity was demonstrated with an on-column loading of 10 ng of vitamin E, which could be clearly detected and identified.Keywords
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