The nucleotide sequence recognized by theEscherichia coliA restriction and modification enzyme
- 1 January 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (2) , 887-899
- https://doi.org/10.1093/nar/12.2.887
Abstract
The nucleotide recognition sequence for the restriction-modification enzyme of Escherichia coli A (EcoA) has been determined to be GAG-7N-GTCA. This sequence is fairly similar, but distinctly different from the two other type I restriction enzyme recognition sites known for E. coli B and E. coli K12, respectively. N6-adenosine methylation has been observed at nucleotide positions 2 and 12 within that sequence after modification by EcoA. As a reference point for mapping the single EcoA site in lambda, the position of lambda point mutation Oam29 has been determined also.Keywords
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