L Organisms of Klieneberger and Streptobacillus Moniliformis

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Abstract
L1 strains which were described by Klieneberger in the cultures of Streptobacillus moniliformis as a symbiont similar in properties to the organism of pleuro-pneumonia bovis, were isolated from the bacillary cultures. Pleuropneumonia-like organisms were also cultivated directly from lesions of white rats (L3). The 2 groups of strains (L1 and L3) show characteristic differences in conformity with the observations of Klieneberger. A strain conforming to the L3 strains was isolated from a human patient. The L1 strains isolated from the Streptobacillus behaved in every respect as a bacterial variant. They appeared in the form of secondary colonies in the bacterial cultures and under appropriate conditions reverted regularly into the typical Streptobacillus moniliformis. The L variant is similar in morphology to bacteria except that it shows unusual pleomorphism and a tendency to produce large round bodies which soon are autolyzed. This, together with the fragility of the organism, is responsible for the special properties of the cultures. The transformation of bacteria to large, fragile round bodies occurs occasionally in different bacterial cultures. This process does not differentiate the pleuropneumonia-like organisms from bacteria. The pleuropneumonia-like organisms are bacterial organisms and the L1 strains are not symbiont in the cultures of Streptobacillus moniliformis but a variant form of the bacillus. A method of staining the fragile colonies of the pleuropneumonia-like organism on the surface of the agar is described.