Studies of the Root-Nodule Organism of Lupinus

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Abstract
Rhizobium lupini (Phytomyxa l. Schroeter) is a Gram-negative organism, producing scant to moderate growth and alkaline reaction on yeast-water mannitol agar, a slightly alkaline reaction without reduction and without formation of a serum zone in litmus milk, and meagre growth on potato and parsnip slopes and on carrot agar. Slight production of tyrosinase by all strains was noted on tyrosine media. Growth and liquefaction on beef-extract peptone gelatin, sensitivity to crystal violet, and nature of nodulation and benefit to host plant varied among different strains; strain variation also occurred in fermentation characters, but results with certain sugars were definite enough to separate R. lupini from other root-nodule bacteria.