Generation of Large Libraries of Random Mutants in Bacillus subtilis by PCR-Based Plasmid Multimerization
Open Access
- 7 August 1997
- journal article
- Published by Taylor & Francis in BioTechniques
- Vol. 23 (2) , 304-310
- https://doi.org/10.2144/97232rr01
Abstract
We describe a PCR-based method for the generation of plasmid multimers that can be directly transformed into Bacillus subtilis with very high efficiency. This technique is particularly useful for t...Keywords
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