Survey of natural occurrence of trichothecene mycotoxins and zearalenone in Korean cereals harvested in 1992 using gas chromatography/mass spectrometry

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Abstract
For the survey of the natural occurrence of trichothecene mycotoxins, produced by species of fungi imperfecti such as Fusarium and Trichothecium a sensitive analytical method was developed for the simultaneous detection and quantitation of the major trichothecene mycotoxins, viz. T‐2 toxin (T‐2), HT‐2 toxin (HT‐2), nivalenol (NIV), fusarenon‐X (F‐X), deoxynivalenol (DON), 3‐acetyl deoxynivalenol (3‐Ac DON), and zearalenone (ZEN), using gas chromatography/mass spectrometry‐selected ion monitoring (GC/MS‐SIM) mode after trimethyl silyl derivatization. The incidences of NIV and DON in 30 barley samples were 93% and 67%, respectively; the average contents of NTV and DON in positive samples were 390 ng/g (range 40–2038) and 106 ng/g (range 5–361) respectively. In 15 maize samples, the incidences of NIV and DON were 53% and 93% respectively and the average contents were 168 ng/g and 145 ng/g, respectively. These results suggest that NIV and DON were the major contaminating trichothecene mycotoxins in Korean barley and maize samples harvested in 1992.

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