Role of a Peptide Tagging System in Degradation of Proteins Synthesized from Damaged Messenger RNA
- 16 February 1996
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 271 (5251) , 990-993
- https://doi.org/10.1126/science.271.5251.990
Abstract
Variants of λ repressor and cytochrome b 562 translated from messenger RNAs without stop codons were modified by carboxyl terminal addition of an ssrA -encoded peptide tag and subsequently degraded by carboxyl terminal-specific proteases present in both the cytoplasm and periplasm of Escherichia coli . The tag appears to be added to the carboxyl terminus of the nascent polypeptide chain by cotranslational switching of the ribosome from the damaged messenger RNA to ssrA RNA.Keywords
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