Determination of Plasma Norepinephrine and Epinephrine by High Performance Liquid Chromatography Using a Two-Column System and an Electrochemical Detector

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Abstract
A method for the simultaneous plasma norepinephrine (NE) and epinephrine (E) determination by reversed-phase ion-pair liquid chromatography with electrochemical detection was developed. Catecholamines were extracted from a 4 ml human plasma sample using an alumina adsorption procedure. A 2 pump, 2-injection valve, 2-column system allowed both to detect plasma NE and E with a good sensitivity due to large injected volumes of extract without any electrochemical detector disturbance and to eliminate uric acid and dopa and low k'' of which would prevent the NE detection. Using this method, NE and E would be detected in respective injected amounts down to 30 and 50 pg. Plasma NE and E determinations were linear in the range of 288-788 pg/ml and 24-274 pg/ml, respectively. The reproducibility, expressed as the coefficients of variation, varied from 2.1% for NE to 10.8% for E.

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