Labelling oligonucleotides to high specific activity (I)
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (12) , 4605-4610
- https://doi.org/10.1093/nar/17.12.4605
Abstract
The normal procedure for labelling oligonucleotides radioactively is the use of polynucleotide kinase and .gamma.32P-ATP. However, this has the disadvantage of only introducing one labelled base per molecule of the oligonucleotide. In this paper we describe an approach based on primer/template combinations using conventional fill-in conditions followed by the release of the labelled sequence by digestion with uracil-DNA glycosylase.This publication has 9 references indexed in Scilit:
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