EVIDENCE FOR PLURIPOTENT STEM-CELL ORIGIN OF IDIOPATHIC MYELOFIBROSIS - CLONAL ANALYSIS OF A CASE CHARACTERIZED BY A N-RAS GENE MUTATION

  • 1 October 1988
    • journal article
    • research article
    • Vol. 2  (10) , 658-660
Abstract
Three cases of idiopathic myelofibrosis were screened for the presence of mutations at codon 12, 13, or 61 of the ras gene family by a rapid method based on polymerase chain reaction and hybridization to mutation-specific oligonucleotides. PB cells of one patient showed a point mutation at codon 12 of the N-ras oncogene. This molecular genetic hallmark was used to investigate the clonal relationship of different cell lineages by cell separation analysis. Presence of the N-ras 12 mutation in granulocytes, monocytes, B cells, and T lymphocytes, as well as erythroblasts, indicates that idiopathic myelofibrosis originates from a pluripotent stem cell, at least in this patient.

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