SYRINGING AS A METHOD OF CELL DISPERSAL .1. EFFECT ON INTERMEDIATE AND SUPERFICIAL SQUAMOUS CELLS

  • 1 January 1978
    • journal article
    • research article
    • Vol. 22  (2) , 86-90
Abstract
Mechanical shear in the form of syringing was applied to human gynecologic cancer cytology specimens in an effort to disperse cell clumps. The percentage of single squamous cells achieved with the optimum conditions is 70-100%. Syringing with a constant pressure device produces a high yield of single squamous cells without the concurrent high cell loss often observed using enzymatic or chemical procedures. Data presented demonstrate that the slide preparation and clump evaluation procedures used for this study yield reliable and reproducible data. Studies varying stroke number and plunger pressure were completed and conditions for maximum dispersal were established.

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