Characterization of a surface antigen ofEimeria nieschulzi (Apicomplexa, Eimeriidae) sporozoites
- 1 January 1989
- journal article
- research article
- Published by Springer Nature in Zeitschrift Fur Parasitenkunde-Parasitology Research
- Vol. 75 (5) , 343-347
- https://doi.org/10.1007/bf00931128
Abstract
A monoclonal antibody (McAb 3C3) reacting with a pellicular antigen ofEimeria nieschulzi sporozoites has been selected among hybridomas produced against this organism by immunofluorescence assay. This antigen has been shown to be located on the zoite surface by immunofluorescence on living organisms. Capping and shedding of antigen-monoclonal antibody immune complexes was observed upon incubation at 37°C. On western immunoblotting, two polypeptides at 22 and 26 kDa were recognized by McAb 3C3, whereas only one polypeptide of 22 kDa was immunoprecipitated by the same antibody after lactoperoxidase surface radio-iodination of sporozoites.This publication has 29 references indexed in Scilit:
- Characterization of a surface antigen of Eimeria tenella sporozoites and synthesis from a cloned cDNA in Escherichia coliMolecular and Biochemical Parasitology, 1988
- Identification of the sporozoite antigens of Eimeria tenellaMolecular and Biochemical Parasitology, 1986
- Control of chicken coccidiosisParasitology Today, 1986
- Effects of Cationized Ferritin and Neuraminidase on Invasion of Cultured Cells by Eimeria meleagrimitis SporozoitesThe Journal of Protozoology, 1984
- Ultrastructural Localization of Monoclonal IgG Antibodies for Antigenic Sites of Eimeria tenella Oocysts, Sporocysts, and Sporozoites1The Journal of Protozoology, 1983
- Direct evidence for microfilament-mediated capping of surface receptors on crawling fibroblastsNature, 1983
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Antibodies to major histocompatibility antigens produced by hybrid cell linesNature, 1977
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Fixation of Ejaculated Spermatozoa for Electron MicroscopyNature, 1967