INHIBITION OF HISTAMINE-SECRETION FROM RAT PERITONEAL MAST-CELLS AND RABBIT LEUKOCYTES BY P-BROMOPHENACYL BROMIDE, A PHOSPHOLIPASE-A2 INHIBITOR

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  • 1 January 1987
    • journal article
    • research article
    • Vol. 55  (1) , 17-24
Abstract
The present investigations was undertaken to explore the role of phospholipase A2 (PLA2) activation in histamine secretion from rabbit leukocytes and rat peritoneal mast cells (RPMC) by using p-bromophenacyl bromide (p-BPB), a potent inhibitor of PLA2. p-BPB exerted concentration dependent inhibition of allergic histamine release from RPMC and rabbit leukocytes as well as calcium ionophore A23187-stimulated histamine release from RPMC with IC50s of 36.2, 1.3 and 80.0 nM, respectively. p-BPB exerted a bell-shaped (biphasic) concentration-effect (inhibition) curve on ragweed antigen-induced histamine secretion from rabbit leukocytes. The data obtained in this study suggest that PLA2 activation plays an essential role in histamine secretion from rabbit leukocytes and RPMC.