Total synthesis of bovine pancreatic ribonuclease A. Part 6. Synthesis of RNase A with full enzymic activity
- 1 January 1981
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Journal of the Chemical Society, Perkin Transactions 1
- p. 831-841
- https://doi.org/10.1039/p19810000831
Abstract
Protected bovine pancreatic RNase A was synthesized by azide condensation of the three fragments Z(OMe)-(RNase 21–124)-OBzl, Z(OMe)-(RNase 9–20)-NHNH2, and Z-(RNase 1–8)-NHNH2. After removal of the protecting groups by methanesulphonic acid, establishment of the disulphide bridges by aerial oxidation, and purification by affinity chromatography and ion-exchange chromatography on CM-cellulose, a synthetic protein was obtained which was indistinguishable by chemical and enzymatic criteria from natural RNase A. Comparable results were also obtained when HF was used as a deprotecting reagent. The unambiguous synthesis of a protein having full enzymic activity has thus been accomplished for the first time.Keywords
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