Complex alternative processing of human cytomegalovirus UL37 pre-mRNA
Open Access
- 1 December 2003
- journal article
- Published by Microbiology Society in Journal of General Virology
- Vol. 84 (12) , 3353-3358
- https://doi.org/10.1099/vir.0.19404-0
Abstract
Differentially processed human cytomegalovirus (HCMV) UL37 RNAs encode biologically significant proteins. Due to the recent discovery of alternative UL37 exon 3 (UL37x3) splice donors, permissively infected cells were thoroughly examined for additional alternatively spliced UL37 RNAs. Newly described donors within UL37 exon 1 (nt 52520) and intron 1 (nt 52209) as well as UL37x3 di (nt 50770) and dii (nt 50782) were differentially spliced to known downstream UL37 acceptors. The alternatively spliced UL37S, UL37L, UL37di and UL37dii RNAs predictably encode proteins of 83, 163, 217 and 213 residues, respectively, which share UL37x1 N-terminal sequences but differ downstream in their C termini. Moreover, temporal expression of the alternatively spliced UL37 RNAs differs during HCMV infection. The complexity of UL37 pre-mRNA processing is evidenced by the detection of 11 UL37 spliced and unspliced UL37x1 RNAs in HCMV-infected cells. Based upon these data, a revised HCMV UL37 gene map is presented, which incorporates all RNA species detected during permissive infection.Keywords
This publication has 13 references indexed in Scilit:
- Disruption of Mitochondrial Networks by the Human Cytomegalovirus UL37 Gene Product Viral Mitochondrion-Localized Inhibitor of ApoptosisJournal of Virology, 2003
- Human cytomegalovirus UL37 immediate early target minigene RNAs are accurately spliced and polyadenylatedJournal of General Virology, 2003
- Novel Immediate-Early Protein IE19 of Human Cytomegalovirus Activates the Origin Recognition Complex I Promoter in a Cooperative Manner with IE72Journal of Virology, 2002
- The carboxyl terminus of the human cytomegalovirus UL37 immediate-early glycoprotein is conserved in primary strains and is important for transactivationJournal of General Virology, 2001
- The Sequence and Antiapoptotic Functional Domains of the Human Cytomegalovirus UL37 Exon 1 Immediate Early Protein Are Conserved in Multiple Primary StrainsVirology, 2001
- Human cytomegalovirus UL37 immediate-early regulatory proteins traffic through the secretory apparatus and to mitochondriaMicrobiology, 2000
- The Acidic Domain of pUL37x1 and gpUL37 Plays a Key Role in Transactivation of HCMV DNA Replication Gene Promoter ConstructionsVirology, 1998
- The human cytomegalovirus US3 immediate-early protein lacking the putative transmembrane domain regulates gene expressionNucleic Acids Research, 1993
- Expression of the human cytomegalovirus UL36–38 immediate early region during permissive infectionVirology, 1991
- An immediate early gene of human cytomegalovirus encodes a potential membrane glycoproteinVirology, 1988