Light-responsivepsbATranscription Requires the −35 Hexamer in the Promoter and Its Proximal Upstream Element, UPE, in Cyanobacteria
- 1 January 2003
- journal article
- Published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry
- Vol. 67 (6) , 1382-1390
- https://doi.org/10.1271/bbb.67.1382
Abstract
We characterized the function of the -35 hexamer in the promoter and an element just upstream, UPE, in the expression in a unicellular cyanobacterium, Microcystis aeruginosa K-81, of the light-responsive gene psbA2, which encodes a reaction center key protein for photosynthesis. A series of mutants with mutations at the -35 hexamer (-35 to -30) and a novel conserved upstream element (UPE: -45 to -36, +1 referring to the transcription start point) were constructed. Expression of the mutants was examined in vivo and in vitro by analyses using a beta-galactosidase assay, primer extension, and a DNA-mobility shift assay with RNA polymerases. Results indicated that the -35 hexamer and its proximal UPE act as effective cis-elements for the light-responsive and/or basal transcription, respectively. A model of the 5'-upstream region with cis- and possible trans-acting factors is presented for the psbA regulatory system.Keywords
This publication has 1 reference indexed in Scilit:
- Gene Structure and Characterization of rpoD and psbA in CyanobacteriaPublished by Springer Nature ,1992