Bodian's Silver Method Stains Neurofilament Polypeptides

Publisher Website
Google Scholar
Abstract
Bodian''s Ag method was used to stain polypeptides of rat spinal cord or peripheral nerve separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The bands corresponding to the 3 polypeptide subunits of the neurofilaments were intensely impregnated. Two other polypeptides were stained inconsistently and less intensely. The tubulin band was stained weakly or not at all; other polypeptides, including glial fibrillary acidic protein, actin and vimentin, remained unstained. This novel application of Bodian''s method provides indirect proof that neurofilaments are the neuronal subcellular structure stained by the technique.