The Activation Function of Steroidogenic Factor-1 is Impaired in Acth-Resistant Y1 Mutants

1 January 2000

journal article
research article
Published by Taylor & Francis in Endocrine Research

Vol. 26 (4) , 1005-1009
https://doi.org/10.3109/07435800009048631

Abstract

This study explores the basis for the altered function of steroidogenic factor-1 (SF1) in a family of ACTH-resistant Y1 adrenal cell mutants. As determined in electrophoretic mobility shift assays, the DNA binding activity of SF1 was not impaired in the mutant clones. Instead, the ability of SF1 to interact with the coactivator, GRIP1 was affected as determined in a modified mammalian 2-hybrid assay. These findings indicate that the mutants harbor a defect affecting the activation function of SF1.

Keywords

This publication has 4 references indexed in Scilit:

Impaired Steroidogenic Factor 1 (NR5A1) Activity in Mutant Y1 Mouse Adrenocortical Tumor Cells
Molecular Endocrinology, 2000
Phosphorylation of the Nuclear Receptor SF-1 Modulates Cofactor Recruitment
Molecular Cell, 1999
Wilms' Tumor 1 and Dax-1 Modulate the Orphan Nuclear Receptor SF-1 in Sex-Specific Gene Expression
Cell, 1998
The Mouse Adrenocorticotropin Receptor Gene: Cloning and Characterization of Its Promoter and Evidence for a Role for the Orphan Nuclear Receptor Steroidogenic Factor 1
Molecular Endocrinology, 1997