Mutation to Herbicide Resistance Maps Within the psbA Gene of Anacystis nidulans R2
- 13 September 1985
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 229 (4718) , 1104-1107
- https://doi.org/10.1126/science.3929379
Abstract
A psbA gene encoding the target of photosystem II herbicide inhibition, the 32,000-dalton thylakoid membrane protein, has been cloned from a mutant of Anacystis nidulans R2, which is resistant to 3-(3,4-dichlorophenyl)-1,1-dimethylurea-(diuron). A cloned DNA fragment from within the coding region of this gene transforms wild-type cells to herbicide resistance, proving that mutation within psbA is responsible for that phenotype. The mutation consists of a single nucleotide change that replaces serine at position 264 of the wild-type protein with alanine in that of the diuron-resistant mutant.Keywords
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