Study of the binding of jatrophone to Escherichia coli s‐ribonucleic acid
- 28 November 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 164 (1) , 51-56
- https://doi.org/10.1016/0014-5793(83)80017-9
Abstract
The interaction of jatrophone with sRNA with sRNA from Escherichia coli has been investigated through UV, CD, and 1H NMR measurements. The results obtained show that the interaction with jatrophone increases the stability of the polynucleotide. It appears that the optical properties of jatrophone depend upon the jatrophone/nucleotide ratio. The observed behaviour can only be explained by the existence of different types of interaction between jatrophone and sRNA. Even for a jatrophone/nucleotide ratio as low as 0.17 the 1H NMR spectra show a multiplicity of resonances that can only be explained by the simultaneous existence of two different binding modes involving the jatrophone molecules.Keywords
This publication has 6 references indexed in Scilit:
- On the interaction between jatrophone and DNAFEBS Letters, 1981
- A strategy for the total synthesis of jatrophone: synthesis of normethyljatrophoneJournal of the American Chemical Society, 1981
- Tumor inhibitors. 111. Structure and stereochemistry of jatrophone, a novel macrocyclic diterpenoid tumor inhibitorJournal of the American Chemical Society, 1976
- Reaction of biological thiols with the tumor inhibitor of jatrophone Inhibition of RNA polymeraseBiochimica et Biophysica Acta (BBA) - Enzymology, 1973
- Jatrophone, a novel macrocyclic diterpenoid tumor inhibitor from Jatropha gossypiifoliaJournal of the American Chemical Society, 1970
- Studies of the binding of ethidium bromide to transfer ribonucleic acid: Absorption, fluorescence, ultracentrifugation and kinetic investigationsJournal of Molecular Biology, 1969