Expression of HGF/SF and Met protein is associated with genetic alterations of VHL gene in primary renal cell carcinomas
- 1 March 2002
- Vol. 110 (3) , 229-238
- https://doi.org/10.1034/j.1600-0463.2002.100305.x
Abstract
We analyzed the genetic alterations of VHL, HGF/SF, and Met genes and the expression pattern of HGF/SF and Met protein in 26 renal cell carcinomas (RCCs). We found five mutations of the VHL gene and frequent LOH (50%) only in non-papillary clear cell RCC. We found six cases in which the CpG island of VHL was methylated. In addition, one missense mutation of the HGF/SF gene was detected in clear cell RCC. HGF/SF and Met protein were expressed in 84.6% and 80.7% of RCCs, respectively. All of the cases with the genetic alterations of VHL or HGF/SF demonstrated strong expression of HGF/SF and Met protein in RCC cells. Statistically, genetic alterations of VHL and HGF/SF were significantly correlated with HGF/SF and Met expression (Fisher's exact test, p=0.022 and p=0.0070). Thus, these results strongly suggest that the expression of HGF/SF and Met protein is closely associated with the genetic alterations of VHL and HGF/SF in primary RCCs.Keywords
This publication has 23 references indexed in Scilit:
- Induction of tissue inhibitor of metalloproteinases-3 is a delayed early cellular response to hepatocyte growth factorOncogene, 1998
- C-Met Proto-Oncogene Expression in Benign and Malignant Human Renal TissuesJournal of Urology, 1997
- Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.Proceedings of the National Academy of Sciences, 1996
- Binding of the von Hippel-Lindau Tumor Suppressor Protein to Elongin B and CScience, 1995
- Inhibition of Transcription Elongation by the VHL Tumor Suppressor ProteinScience, 1995
- Silencing of the VHL tumor-suppressor gene by DNA methylation in renal carcinoma.Proceedings of the National Academy of Sciences, 1994
- Invasiveness and metastasis of NIH 3T3 cells induced by Met-hepatocyte growth factor/scatter factor autocrine stimulation.Proceedings of the National Academy of Sciences, 1994
- Mutations of the VHL tumour suppressor gene in renal carcinomaNature Genetics, 1994
- The Sensitivity of Single-Strand Conformation Polymorphism Analysis for the Detection of Single Base SubstitutionsGenomics, 1993
- Identification of a fibroblast-derived epithelial morphogen as hepatocyte growth factorPublished by Elsevier ,1991