Subgross Pathology of the Human Breast: Method and Initial Observations2

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Abstract
A method is described for study of the subgross anatomy of entire human breasts obtained at surgery or autopsy. The tissue is serially sectioned at 1–2 mm, defatted in acetone, hydrated, stained 3 hours in hematoxylin at pH 1, dehydrated, cleared in toluene, and stored in sealed plastic bags containing methyl salicylate. Lesions may be studied at low magnifications and photographed without being taken from the bags. Subsequently, the bags can be opened and selected parts of the specimen removed for the preparation of ordinary microscopic slides. Thus light microscopy at the histologic level can be correlated with subgross anatomy. The method offers the advantages of convenience, simplicity, and reliability. It permits quantitative and statistical approaches to the study of focal dysplastic and anaplastic lesions in the human mammary gland.

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