The appearance of single-ion channels in unmodified lipid bilayer membranes at the phase transition temperature

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Abstract
Ion-specific channels in artificial membranes have been formed by the addition of gramicidin A1, alamethicin2, polyene antibiotics3 and some proteins4 to the solution surrounding the bilayer lipid membrane. Until now there have been no reports of single-ion channels in unmodified lipid membranes. We have now studied the electrical conductance of planar lipid bilayers membranes made of synthetic distearoylphosphorylcholine (DSPC). Current fluctuations of amplitude ∼1pA and duration ∼1s have been discovered at phase transition temperature, which shows that the appearance of ionic channels may be the result of lipid domain interactions. This would explain the dramatic increase in ion permeability observed in liposomes during phase transition. We suggest that these channels could conduct the transmembrane ionic current in biological membranes without the involvement of peptides and proteins.