Purification of Human Monocytes on Microexudatecoated Surfaces
Open Access
- 1 April 1978
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 120 (4) , 1372-1374
- https://doi.org/10.4049/jimmunol.120.4.1372
Abstract
Human blood monocytes, but not other Ficoll-Hypaque cells, adhere to plastic surfaces from which confluent BHK cells have been removed. EDTA (3 mM) rapidly and completely removes monocytes, providing a simple technique for preparation of human monocytes in suspension. The method should be valuable for studies of monocyte function where monocyte monolayers are not suitable.Keywords
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