Mitochondrial Histone-Like DNA-Binding Proteins Are Essential for Normal Cell Growth and Mitochondrial Function in Crithidia fasciculata
- 1 April 2004
- journal article
- Published by American Society for Microbiology in Eukaryotic Cell
- Vol. 3 (2) , 518-26
- https://doi.org/10.1128/ec.3.2.518-526.2004
Abstract
The Crithidia fasciculata KAP2 and KAP3 proteins are closely related kinetoplast-specific histone-like DNA-binding proteins. The KAP2 and KAP3 genes are 46% identical and are arranged in tandem on the chromosomal DNA. Disruption of both alleles of either gene alone shows no detectable phenotype. However, replacement of both copies of the sequence encoding the entire KAP2 and KAP3 locus increases maxicircle mRNA levels two- to fourfold. These double-knockout cells are viable but grow extremely slowly, have reduced respiration and very abnormal cell morphologies, and accumulate numerous large vacuoles. The extreme phenotype of these mutant cells suggests an important role for the KAP2 and KAP3 proteins in mitochondrial metabolism and cell growth.Keywords
This publication has 23 references indexed in Scilit:
- Sequence Elements in both the Intergenic Space and the 3′ Untranslated Region of the Crithidia fasciculata KAP3 Gene Are Required for Cell Cycle Regulation of KAP3 mRNAEukaryotic Cell, 2003
- Kinetoplast DNA Network: Evolution of an Improbable StructureEukaryotic Cell, 2002
- A Trypanosome Mitochondrial RNA Polymerase Is Required for Transcription and ReplicationPublished by Elsevier ,2002
- Stable transformation of trypanosomatids through targeted chromosomal integration of the selectable marker gene encoding blasticidin S deaminaseFEMS Microbiology Letters, 2000
- The topology of the kinetoplast DNA networkCell, 1995
- Kinetoplast DNA minicircles encode guide RNAs for editing of cytochrome oxidase subunit III mRNACell, 1990
- Extensive editing of the cytochrome c oxidase III transcript in Trypanosoma bruceiCell, 1988
- Editing of kinetoplastid mitochondrial mRNAs by uridine addition and deletion generates conserved amino acid sequences and AUG initiation codonsCell, 1988
- Major transcript of the frameshifted coxll gene from trypanosome mitochondria contains four nucleotides that are not encoded in the DNACell, 1986
- Kinetoplast DNA, mitochondria DNA with a differenceMolecular and Biochemical Parasitology, 1983