Antigens produced in plants by infection with chimeric plant viruses immunize against rabies virus and HIV-1
- 27 May 1997
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 94 (11) , 5784-5788
- https://doi.org/10.1073/pnas.94.11.5784
Abstract
The coat protein (CP) of alfalfa mosaic virus was used as a carrier molecule to express antigenic peptides from rabies virus and HIV. The antigens were separately cloned into the reading frame of alfalfa mosaic virus CP and placed under the control of the subgenomic promoter of tobacco mosaic virus CP in the 30BRz vector. The in vitro transcripts of recombinant virus with sequences encoding the antigenic peptides were synthesized from DNA constructs and used to inoculate tobacco plants. The plant-produced protein (virus particles) was purified and used for immunization of mice. Both antigens elicited specific virus-neutralizing antibodies in immunized mice.Keywords
This publication has 32 references indexed in Scilit:
- Purification, characterization, assembly and crystallization of assembled alfalfa mosaic virus coat protein expressed in Escherichia coliJournal of General Virology, 1996
- Does the Key to a Successful HIV Type 1 Vaccine Lie among the Envelope Sequences of Infected Individuals?AIDS Research and Human Retroviruses, 1995
- Plant virus expressing hybrid coat protein with added murine epitope elicits autoantibody responseVaccine, 1995
- Human Immunodeficiency Virus Type 1-Neutralizing Antibodies Raised to a Glycoprotein 41 Peptide Expressed on the Surface of a Plant VirusAIDS Research and Human Retroviruses, 1995
- Malaria Epitopes Expressed on the surface of Recombinant Tobacco Mosaic VirusNature Biotechnology, 1995
- A New Tobacco Mosaic Virus Vector and its Use for the Systemic Production of Angiotensin-I-Converting Enzyme Inhibitor in Transgenic Tobacco and TomatoNature Biotechnology, 1993
- Multiple presentation of foreign peptides on the surface of an RNA-free spherical bacteriophage capsidJournal of General Virology, 1993
- Multiple display of foreign peptides on a filamentous bacteriophageJournal of Molecular Biology, 1991
- Filamentous Fusion Phage: Novel Expression Vectors That Display Cloned Antigens on the Virion SurfaceScience, 1985
- Synthesis, Accumulation and Encapsidation of Individual Brome Mosaic Virus RNA Components in Barley ProtoplastsJournal of General Virology, 1980