Evaluation of enzyme-linked immunosorbent assay for the serodiagnosis of amebiasis

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Abstract
This report describes the development and evaluation of an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to Entamoeba histolytica. Highly sensitive and reproducible results were obtained in antigen-coated plates prepared by air-drying at 37.degree. C. Comparison of the ELISA with indirect fluorescent antibody and indirect hemagglutination techniques showed that the former was slightly more sensitive than the 2 latter methods. The specificity was evaluated by testing specially chosen population groups. ELISA was negative in 96.4% of 693 normal adults and children and in 96.6% of 377 patients with various parasitic, bacterial, mycotic and other clinical disease. The assay was positive in 26% of 461 patients with suspected amebiasis and in all of 53 patients with amoebic liver abscess. The ELISA was specific, highly sensitive and reliable for detecting anti-E. histolytica antibodies in humans.

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