Determination of Trace Levels of Hexachlorocyclopentadiene and Octachlorocyclopentene in Body Fluids

Abstract
A simple, rapid and sensitive method for the analysis of hexachlorocyclopentadiene (C5Cl6) and octachlorocyclopentene (C5Cl8) in blood and urine is described. The procedure requires 5.0 mL of blood or urine, and involves the isolation of the compounds from the sample by liquid-liquid extraction, followed by screening and quantification via glass-capillary column gas chromatography with electron-capture detection and confirmation by gas chromatography-mass spectrometry. The assays are suitable for the detection and identification of nanogram quantities of these compounds in body fluids with a lower detection limit of 50 ng/mL for blood and 10 ng/mL for urine.

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