Pichia acaciae Killer System: Genetic Analysis of Toxin Immunity
- 1 July 2007
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 73 (13) , 4373-4378
- https://doi.org/10.1128/aem.00271-07
Abstract
The gene responsible for self-protection in the Pichia acaciae killer plasmid system was identified by heterologous expression in Saccharomyces cerevisiae. Resistance profiling and conditional toxin/immunity coexpression analysis revealed dose-independent protection by pPac1-2 ORF4 and intracellular interference with toxin function, suggesting toxin reinternalization in immune killer cells.Keywords
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