The effect of Streptomycin on antibody synthesis in vitro.

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Abstract
Spleen and lymph node cells from rabbits immunized with the RNA phase MS-2 actively synthesized specific neutralizing and complement-fixing antibody. In the presenc? of 200 [mu]g/ml SM [streptomycin], the appearance of neutralizing activity was reversibly inhibited, but the production of complement-fixing activity was unchanged. Antibody synthesized by untreated cells consistently neutralized closely related RNA phages in the same order observed with serum antibody against MS-2. In the presence of SM the related phages were not neutralized in proportion to the closeness of their relation to MS-2, and the relative order of neutralization varied randomly from trial to trial. It is suggested that SM causes the synthesis of antibody with altered serological specificity in a manner similar to its effect on protein synthesis in bacterial extracts.