Synthesis and Application of Two Reagents for the Introduction of Sulfhydryl Groups into Proteins

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Abstract
Two reagents are described which can be used for the introduction of sulfhydryl groups into proteins. Mercaptopropionylhydrazide modifies specifically periodate‐oxidized N termini of proteins, provided that the N‐terminal residue is serine or threonine. 3‐(Phenyldithio) propionimidate introduces a disulfide bond at lysine residues of proteins. Reduction converts the disulfide into a sulfhydryl group. The imidate compound was found to react with a high specificity with only one lysine residue of ribosomal protein L7/L12.