Studies on 3‐Deoxy‐d‐arabinoheptulosonate‐7‐phosphate Synthetase(phe) from Escherichia coli K12

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Abstract
3-Deoxy-D-arabinoheptulosonate-7-phosphate synthetase(phe) [EC 4.1.2.15] from E. coli K12 was purified to near homogeneity. The purified enzyme has a specific activity of 67 units/mg which is .apprx. 1000 times that found in cell-free extracts of wild-type E. coli K12. The minimum MW of the enzyme was 33,000 by dodecylsulfate-gel electrophoresis. Re-estimation of the native MW by gel filtration confirmed the previously determined value of 110,000. Amino acid analysis and tryptic fingerprints indicated that the subunits of the enzyme are similar and possibly identical. The purified enzyme does not contain Co2+.