Rapid microwave-stimulated fixation of fine-needle aspiration biopsies for transmission electron microscopy
- 1 January 1990
- journal article
- research article
- Published by Wiley in Diagnostic Cytopathology
- Vol. 6 (1) , 68-71
- https://doi.org/10.1002/dc.2840060115
Abstract
A new technique is described in which fine‐needle aspiration biopsies are processed for transmission electron microscopy within 2 hr. This rapid processing method includes exposure of the biopsy in a glutaraldehyde‐formaldehyde mixture to microwaves for 25 sec in a domestic oven. The specimen is then routinely stained, processed through graded ethanol, and embedded in epoxy resin to produce excellent preservation of fine structural features.Keywords
This publication has 16 references indexed in Scilit:
- A Rapid and Reliable Silver Impregnation Method for Pneumocystis Carinii and FungiJournal of Histotechnology, 1988
- Staining Pneumocystis Carinii and Fungi in Unfixed Specimens with Ammoniacal Silver Using a Microwave OvenJournal of Histotechnology, 1988
- Modification of Kluver-Barrera Method for Staining Myelin and Nerve Cells by Rapid MicrowavingLaboratory Medicine, 1988
- Modified Steiner Procedure for Microwave Staining of Spirochetes and Nonfilamentous BacteriaJournal of Histotechnology, 1987
- A method of rapid fixation of large biopsy specimens using microwave irradiationPathology, 1986
- Ultrafast microwave energy fixation for electron microscopy.Journal of Histochemistry & Cytochemistry, 1986
- Microwave‐stimulated diffusion for fast processing of tissue: reduced dehydrating, clearing, and impregnating timesHistopathology, 1986
- Silver-Staining of Senile Plaques and Neurofibrillary Change in Paraffin-Embedded TissuesJournal of Histotechnology, 1985
- Microwave irradiation as a form of fixation for light and electron microscopyThe Journal of Pathology, 1985
- Rapid Metallic Histological Staining Using the Microwave OvenJournal of Histotechnology, 1983