Genetic Characterization of Cryptosporidium Isolates From Ringed Seals (Phoca hispida) in Northern Quebec, Canada
- 1 June 2005
- journal article
- Published by American Society of Parasitologists in Journal of Parasitology
- Vol. 91 (3) , 712-716
- https://doi.org/10.1645/ge-3438rn
Abstract
This study reports the molecular characterization of Cryptosporidium spp. isolates identified from intestinal contents of ringed seals (Phoca hispida) from Nunavik (Quebec, Canada). Cryptosporidium spp. fragments of 18S rRNA, HSP-70, and actin loci were amplified by PCR from seal intestinal contents. PCR-positive specimens were sequenced and compared with other Cryptosporidium species and genotypes reported previously. Sequence analysis showed the presence of C. muris and 2 novel genotypes in ringed seals.Keywords
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