Retransformation of a simian virus 40 revertant cell line, which is resistant to viral and DNA infections, by microinjection of viral DNA

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Abstract
Morphological transformants of cultured cells were isolated as dense foci on a monolayer of normal cells approximately 4 wk after microinjection of purified SV-40 DNA (200-400 molecules/cell) directly into the nucleus. Rat 1 (an established contact-inhibited rat embryo fibroblast line) and F1'' 1-4 (a 5-fluorodeoxyuridine-selected flat revertant from the SV-40-transformed 14B cell line) were transformed with an efficiency of 5-10% of the cells injected. F1'' 1-4 is not susceptible to retransformation by viral or DNA infection (by calcium phosphate-facilitated cellular uptake), and as a result it was previously thought to possess a host mutation preventing expression of the SV-40 genome.