Demonstration of antibodies against Yersinia enterocolitica lipopolysaccharide in human sera by enzyme-linked immunosorbent assay
- 1 September 1979
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 10 (3) , 279-284
- https://doi.org/10.1128/jcm.10.3.279-284.1979
Abstract
Antibodies against Y. enterocolitica serotype O:3 lipopolysaccharide [LPS] present in sera from patients with Yersinia infection were studied using an enzyme-linked immunosorbent assay. Of the sera with significant bacterial agglutination titers against Y. enterocolitica type O:3, 86% contained anti-LPS IgG antibodies. The sera of some patients demonstrated increasing anti-LPS IgG antibody levels despite decreasing bacterial agglutination titers. The assay was specific for Y. enterocolitica type O:3 LPS and in inhibition experiments LPS could be detected in amounts of .gtoreq. 0.5 .mu.g/ml.This publication has 21 references indexed in Scilit:
- The enzyme-linked immunosorbent assay (ELISA): A measure of antibody concentration or affinity?Immunochemistry, 1978
- A Solid Phase Enzyme‐linked Immunosorbent Assay (ELISA) for the Demonstration of Antibodies against Denatured, Single‐stranded DNA in Patient SeraScandinavian Journal of Immunology, 1978
- Enzyme-immunoassayClinica Chimica Acta; International Journal of Clinical Chemistry, 1977
- Relation Between HLA‐B27 and Clinical Features in Patients with Yersinia ArthritisArthritis & Rheumatism, 1977
- ENZYME‐LINKED IMMUNOSORBENT ASSAY (ELISA) FOR TITRATION OF ANTIBODIES AGAINST BRUCELLA ABORTUS AND YERSINIA ENTEROCOLITICAActa Pathologica Microbiologica Scandinavica Section C Immunology, 1976
- Quantitation of Salmonella O-Antibodies in Human Sera by Enzyme-Linked Immunosorbent Assay (ELISA)International Archives of Allergy and Immunology, 1975
- HL-A, IMMUNE-RESPONSE GENES, AND DISEASEPublished by Elsevier ,1974
- Picogram-sensitive assay for endotoxin: Gelation of Limulus polyphemus blood cell lysate induced lipopolysaccharides and lipid A from gram-negative bacteriaBiochimica et Biophysica Acta (BBA) - General Subjects, 1972
- Enzyme-linked immunosorbent assay. II. Quantitative assay of protein antigen, immunoglobulin g, by means of enzyme-labelled antigen and antibody-coated tubesBiochimica et Biophysica Acta (BBA) - Protein Structure, 1971
- Yersinia enterocolitica (Pasteurella x) in human enteric infections.BMJ, 1966